Quantitative Proteomic Analysis of Histone Modifications
نویسندگان
چکیده
منابع مشابه
Quantitative Proteomic Approaches to Studying Histone Modifications
Histone post-translational modifications (PTMs) positively and negatively regulate gene expression, and are consequently a vital influence on the genomic profile of all eukaryotic species. The study of histone PTMs using classical methods in molecular biology, such as immunofluorescence and Western blotting, is challenging given the technical issues of the approaches, and chemical diversity and...
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RNA modifications impact numerous cellular processes such as pre-mRNA splicing and protein synthesis. The elucidation of the mechanisms by which these modifications impact cellular processes necessitates the ability to both detect and quantify the presence of these modifications within RNA molecules. Here, we present a detailed procedure that allows the detection and quantification of RNA base ...
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ژورنال
عنوان ژورنال: Chemical Reviews
سال: 2015
ISSN: 0009-2665,1520-6890
DOI: 10.1021/cr500491u